Protein synthesis during fungal spore germination. IV. Transfer ribonucleic acid from germinated and ungerminated spores.
نویسندگان
چکیده
Transfer ribonucleic acid (tRNA) fractions isolated from germinated and ungerminated spores of Botryodiplodia theobromae and Rhizopus stolonifer had acceptor activity for all 20 amino acids commonly found in protein, when tested with an enzyme fraction from germinated spores. Accordingly, it is unlikely that the absence of tRNA for a particular amino acid limits protein synthesis in fungal spores.
منابع مشابه
Protein synthesis during fungal spore germination. II. Aminoacyl-soluble ribonucleic acid synthetase activities during germination of Botryodiplodia theobromae spores.
The specific activities of 13 aminoacyl-soluble ribonucleic acid (sRNA) synthetases were measured at various time intervals during the germination of Botryodiplodia theobromae conidiospores. The enzyme activities were low or absent in ungerminated spores, and they increased rapidly as germination proceeded. When extracts of the ungerminated spores were prepared with mortar and pestle, very litt...
متن کاملProtein synthesis during fungal spore germination: differential protein synthesis during germination of Botryodiplodia theobromae spores.
The preformed messenger ribonucleic acid in Botryodiplodia theobromae spores directs the synthesis of several relatively stable polypeptides
متن کاملGermination of Neurospora crassal
Transfer ribonucleic acid (tRNA) methylases were studied during the germination of spores in Neurospora crassa. The total methylase capacity and base specific tRNA methylase activities were determined in extracts from cells harvested at various stages of germination. Germinated conidia have a 65% higher methylase capacity than ungerminated conidia. Three predominant methylase activities were fo...
متن کاملA microtiter fluorometric assay to detect the germination of Bacillus anthracis spores and the germination inhibitory effects of antibodies.
Bacillus anthracis spore germination is usually detected in vitro by alterations in spore refractility, heat resistance, and stainability. We developed a more quantitative, sensitive, and semi-automated procedure for detecting germination by using a microtiter kinetic reader for fluorescence spectrophotometry. The procedure was based on the increase in fluorescence of spores with time during th...
متن کاملPolysomes, ribonucleic acid, and protein synthesis during germination of Neurospora crassa conidia.
The level of polysomes in ungerminated conidia of Neurospora crassa depends on the method used to collect spores. Spores harvested and exposed to hydration contain 30% of their ribosomes as polysomes, whereas those not exposed to hydration contain only 3% of their ribosomes as polysomes. During the germination process, the percentage of the ribosomes which sediment as polysomes increases rapidl...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of bacteriology
دوره 100 3 شماره
صفحات -
تاریخ انتشار 1969